In:
Scientific Reports, Springer Science and Business Media LLC, Vol. 7, No. 1 ( 2017-11-21)
Abstract:
Hepatitis C virus (HCV), dengue virus (DENV) and Japanese encephalitis virus (JEV) belong to the family Flaviviridae . Their viral particles have the envelope composed of viral proteins and a lipid bilayer acquired from budding through the endoplasmic reticulum (ER). The phospholipid content of the ER membrane differs from that of the plasma membrane (PM). The phospholipase A 2 (PLA 2 ) superfamily consists of a large number of members that specifically catalyse the hydrolysis of phospholipids at a particular position. Here we show that the CM-II isoform of secreted PLA 2 obtained from Naja mossambica mossambica snake venom (CM-II-sPLA 2 ) possesses potent virucidal (neutralising) activity against HCV, DENV and JEV, with 50% inhibitory concentrations (IC 50 ) of 0.036, 0.31 and 1.34 ng/ml, respectively. In contrast, the IC 50 values of CM-II-sPLA 2 against viruses that bud through the PM (Sindbis virus, influenza virus and Sendai virus) or trans -Golgi network (TGN) (herpes simplex virus) were 〉 10,000 ng/ml. Moreover, the 50% cytotoxic (CC 50 ) and haemolytic (HC 50 ) concentrations of CM-II-sPLA 2 were 〉 10,000 ng/ml, implying that CM-II-sPLA 2 did not significantly damage the PM. These results suggest that CM-II-sPLA 2 and its derivatives are good candidates for the development of broad-spectrum antiviral drugs that target viral envelope lipid bilayers derived from the ER membrane.
Type of Medium:
Online Resource
ISSN:
2045-2322
DOI:
10.1038/s41598-017-16130-w
Language:
English
Publisher:
Springer Science and Business Media LLC
Publication Date:
2017
detail.hit.zdb_id:
2615211-3
