In:
Experimental & Molecular Medicine, Springer Science and Business Media LLC, Vol. 54, No. 12 ( 2022-12-09), p. 2188-2199
Abstract:
The generation of conditional alleles using CRISPR technology is still challenging. Here, we introduce a Short Conditional intrON (SCON, 189 bp) that enables the rapid generation of conditional alleles via one-step zygote injection. In this study, a total of 13 SCON mouse lines were successfully generated by 2 different laboratories. SCON has conditional intronic functions in various vertebrate species, and its target insertion is as simple as CRISPR/Cas9-mediated gene tagging.
Type of Medium:
Online Resource
ISSN:
2092-6413
DOI:
10.1038/s12276-022-00891-0
Language:
English
Publisher:
Springer Science and Business Media LLC
Publication Date:
2022
detail.hit.zdb_id:
2084833-X