In:
FEBS Letters, Wiley, Vol. 351, No. 3 ( 1994-09-12), p. 385-388
Abstract:
The cyoE gene of the Escherichia coli bo ‐type quinol oxidase operon ( cyo ABCDE ) has been previously shown to encode heme O synthase. To demonstrate a catalytic role of a cyoE homologue (the caaE gene) in the gene cluster for caa 3 ‐type cytochrome c oxidase of thermophilic Bacillus PS3, we have carried out genetic complementation analysis using the chimeric operon cyo ABCD‐caaE and heme O synthase assay using the CaaE‐overproduced E. coli membranes. We found that the caaE gene encodes a thermotolerant heme O synthase which provides an intermediate for heme A biosynthesis.
Type of Medium:
Online Resource
ISSN:
0014-5793
,
1873-3468
DOI:
10.1016/0014-5793(94)00889-2
Language:
English
Publisher:
Wiley
Publication Date:
1994
detail.hit.zdb_id:
1460391-3
SSG:
12