In:
FEBS Letters, Wiley, Vol. 162, No. 2 ( 1983-10-17), p. 406-410
Abstract:
ATP‐dependent calcium uptake was studied in isolated guinea pig parotid gland microsomes. The apparent K m for free Ca 2+ was 0.41 μM, the apparent K m for ATP·Mg 2− 0.23 mM. The pH optimum was 6.8–7.0. Subfractionation of the microsomes revealed that the highest specific uptake activity resided in a rather dense fraction of the endoplasmic reticulum. The calcium uptake/ATPase stoichiometry was determined in the absence of exogenous magnesium in the submicrosomal fractions. It ranged from 1–2. It is concluded that in vivo the stoichiometry is the same as in sarcoplasmic reticulum, namely 2.
Type of Medium:
Online Resource
ISSN:
0014-5793
,
1873-3468
DOI:
10.1016/0014-5793(83)80796-0
Language:
English
Publisher:
Wiley
Publication Date:
1983
detail.hit.zdb_id:
1460391-3
SSG:
12