In:
Bioscience Reports, Portland Press Ltd., Vol. 25, No. 5-6 ( 2005-10-12), p. 387-397
Abstract:
The effect of exogenous cytochrome c on respiration rate of the rat and human heart mitochondria was assessed in situ, using permeabilized fibers. It was (i) much more pronounced in State 2 and 4 than in State 3 with all the respiratory substrates (pyruvate+malate, succinate, palmitoyl-CoA+carnitine and octanoyl-L-carnitine), (ii) different with different substrates, (iii) much higher after ischemia in both metabolic states, particularly in the case of succinate oxidation compared to pyruvate+malate, (iv) the highest in State 4 with succinate as a substrate. Similar results were obtained with the isolated rat and rabbit heart mitochondria. The differences in the degree of stimulation of mitochondrial respiration by cytochrome c and, thus, sensitivity of cytochrome c test in evaluation of the intactness/injury of outer mitochondrial membrane are probably determined by the differences in the cytochrome c role in the control of mitochondrial respiration in the above-described conditions.
Type of Medium:
Online Resource
ISSN:
0144-8463
,
1573-4935
DOI:
10.1007/s10540-005-2897-2
Language:
English
Publisher:
Portland Press Ltd.
Publication Date:
2005
detail.hit.zdb_id:
764946-0
detail.hit.zdb_id:
2014993-1
SSG:
12
