In:
Reproductive Medicine and Biology, Wiley, Vol. 17, No. 2 ( 2018-04), p. 155-163
Abstract:
The effect of supplementing maturation medium with follicular fluid ( FF ) was examined according to its non‐esterified fatty acid ( NEFA ) content or with a fatty acid mixture ( FA ‐Mix) on the developmental competence of oocytes, as well as the mitochondrial quality and quantity in the oocytes and cumulus cells. Method Porcine oocytes from a slaughterhouse were used. Results The FF or FA ‐Mix in maturation medium increased the lipid content in both the oocytes and the cumulus cells, but the adenosine triphosphate content was differentially affected. The FF supplementation increased the mitochondrial DNA copy number, survival of cumulus cells, and rate of oocyte development to the blastocyst stage, whereas the FA ‐Mix supplementation did not show these effects. The expression levels of GPC 4 , PFKP , PRDX 3 , and TFAM in the cumulus cells increased after FF supplementation, but the expression of GJA 1 decreased, compared with the cells that were cultured without FF . Conclusion Adding FF and FA ‐Mix to the maturation medium increased the lipid content in the oocytes and cumulus cells. The effects of FF on the cumulus cells and oocytes were not observed after FA ‐Mix supplementation, indicating that the concentration of the NEFA s in the FF are closely associated with an ability to support oocyte maturation and the metabolism of cumulus cells and oocytes.
Type of Medium:
Online Resource
ISSN:
1445-5781
,
1447-0578
Language:
English
Publisher:
Wiley
Publication Date:
2018
detail.hit.zdb_id:
2081579-7
