In:
Rapid Communications in Mass Spectrometry, Wiley, Vol. 15, No. 19 ( 2001-10-15), p. 1832-1839
Abstract:
An Erratum for this article has been published in Rapid Communications in Mass Spectrometry 17(3) 2003, 264 Biotransformation studies performed on an investigational compound (I, represented by R1‐CH(NH 2 )‐CO‐N(R2)‐CH 2 ‐S‐R3) led to the identification of five metabolites (M1–M5). Based on LC/MS (liquid chromatography/mass spectrometry) analysis which included the use of H 2 O and D 2 O in the mobile phases, they were identified as the sulfoxide (M1), sulfone (M2), carbamoyl glucuronide (M3), N‐glucuronide (M4), and N‐glucoside (M5) metabolites, respectively. The structure of M3, a less commonly seen carbamoyl glucuronide metabolite, was established using on‐line H/D (hydrogen/deuterium) exchange experiments conducted by LC/MS. H/D exchange experiments were also used to distinguish the S‐oxidation structures of M1 and M2 from hydroxylation. Herein, the application of deuterium oxide as the LC/MS mobile phase for structural elucidation of drug metabolites in biological matrices is demonstrated. Copyright © 2001 John Wiley & Sons, Ltd.
Type of Medium:
Online Resource
ISSN:
0951-4198
,
1097-0231
Language:
English
Publisher:
Wiley
Publication Date:
2001
detail.hit.zdb_id:
2002158-6
detail.hit.zdb_id:
58731-X
SSG:
11
