In:
Pediatric Pulmonology, Wiley, Vol. 57, No. 9 ( 2022-09), p. 2189-2198
Abstract:
In this pilot study, we investigated whether induced sputum (IS) could serve as a viable alternative to bronchoalveolar lavage (BAL) and yield robust inflammatory biomarkers in toddlers with cystic fibrosis (CF) featuring minimal structural lung disease. Methods We collected IS, BAL (right middle lobe and lingula), and blood, and performed chest computed tomography (CT) scans from 2‐year‐olds with CF ( N = 11), all within a single visit. Inflammatory biomarkers included 20 soluble immune mediators and neutrophil elastase (NE), as well as frequency and phenotype of T cells, monocytes/macrophages, and neutrophils. Results At the molecular level, nine mediators showed similar levels in IS and BAL (CXCL1, CXCL8, IL‐1α, IL‐1RA, IL‐6, CCL2, CXCL10, M‐CSF, VEGF‐A), four were higher in IS than in BAL (CXCL5, IL‐1β, CXCL11, TNFSF10), and two were present in IS, but undetectable in BAL (IL‐10, IFN‐γ). Meanwhile, soluble NE had lower activity in IS than in BAL. At the cellular level, T‐cell frequency was lower in IS than in BAL. Monocytes/macrophages were dominant in IS and BAL with similar frequencies, but differing expression of CD16 (lower in IS), CD115, and surface‐associated NE (higher in IS). Neutrophil frequency and phenotype did not differ between IS and BAL. Finally, neutrophil frequency in IS correlated positively with air trapping. Conclusions IS collected from 2‐year‐olds with CF yields biomarkers of early airway inflammation with good agreement with BAL, notably with regard to molecular and cellular outcomes related to neutrophils and monocytes/macrophages.
Type of Medium:
Online Resource
ISSN:
8755-6863
,
1099-0496
Language:
English
Publisher:
Wiley
Publication Date:
2022
detail.hit.zdb_id:
1491904-7
