In:
Obesity, Wiley, Vol. 32, No. 8 ( 2024-08), p. 1441-1447
Abstract:
The aim of this study was to demonstrate that an adipocyte tissue‐derived conditioned medium (ACM) contains inflammatory molecules that induce senescence in B cells. Methods We incubated blood‐derived B cells from lean donors with ACM obtained from the adipose tissue of adult female donors with obesity undergoing weight reduction surgery or with medium as control. After 24 h, cells were harvested, and the expression of transcripts for proinflammatory cytokines (TNF/IL‐6), chemokines (IL‐8), and for markers of the senescence‐associated secretory phenotype (SASP) was measured by quantitative polymerase chain reaction. B cells were also stained with the marker of immunosenescence β‐galactosidase, and their metabolic status was evaluated in Seahorse using a Mito Stress Test. Results We show that the incubation of B cells from lean donors with ACM induces the expression of transcripts for inflammatory and SASP transcripts, increases the amount of β‐galactosidase staining, and induces a metabolic phenotype characterized by higher basal and maximal oxygen consumption, spare respiratory capacity (difference between maximal and basal respiration), nonmitochondrial oxygen consumption, ATP production, and proton leak. Conclusions These results demonstrate that B cells from lean individuals, after incubation with ACM, become inflammatory and senescent, and this occurs through metabolic pathways needed to support their secretory phenotype.
Type of Medium:
Online Resource
ISSN:
1930-7381
,
1930-739X
Language:
English
Publisher:
Wiley
Publication Date:
2024
detail.hit.zdb_id:
2230457-5
detail.hit.zdb_id:
2027211-X
