In:
Molecular Reproduction and Development, Wiley, Vol. 73, No. 12 ( 2006-12), p. 1523-1530
Abstract:
Insulin‐like growth factor (IGF)‐I is a receptor‐mediated autocrine and/or paracrine growth and/or survival factor for mammalian embryo development. It is known to promote the growth and development of mouse preimplantation embryos. The present study was designed to investigate the effects of IGF‐I (50 ng/ml), anti‐IGF‐I receptor antibody (50 ng/ml) and their combination on porcine preimplantation embryo development. Furthermore, the mechanism underlying the embryotropic effects of IGF‐I was evaluated by monitoring the incidence of apoptosis and expression of apoptosis‐related genes. In both in vitro fertilized (IVF) and somatic cell nuclear transfer (SCNT) embryos, culturing with IGF‐I increased the rate of blastocyst formation and this embryotrophic effect was neutralized by culturing with IGF‐I along with anti‐IGF‐I receptor (IGF‐IR) antibody. Culturing IVF and SCNT embryos with IGF‐I significantly increased the number of total cells in blastocysts and decreased the number of apoptotic nuclei. These effects of IGF‐I were also neutralized by culturing with IGF‐I along with anti‐IGF‐IR antibody. Expression of the anti‐apoptotic Bcl‐2 gene was increased, while expression of the pro‐apoptotic Bax was decreased in both IVF and SCNT embryos cultured with IGF‐I. In both IVF and SCNT embryos, anti‐IGF‐IR antibody along with IGF‐I neutralized the effect of IGF‐I on expression of Bcl‐2 and Bax genes. In conclusion, the present study demonstrated that IGF‐I through its specific receptors improved the developmental competence of IVF and SCNT embryos by decreasing the incidence of apoptosis and regulating apoptosis‐related genes in porcine preimplantation embryos. Mol. Reprod. Dev. 73: 1523–1530, 2006. © 2006 Wiley‐Liss, Inc.
Type of Medium:
Online Resource
ISSN:
1040-452X
,
1098-2795
Language:
English
Publisher:
Wiley
Publication Date:
2006
detail.hit.zdb_id:
20321-X
detail.hit.zdb_id:
1493888-1
SSG:
12
