In:
Clinical & Translational Immunology, Wiley, Vol. 7, No. 1 ( 2018-01)
Abstract:
Innate lymphoid cells ( ILC s) share many characteristics with CD 4 + T cells, and group 1 ILC s share a requirement for T‐bet and the ability to produce IFN γ with T helper 1 (Th1) cells. Given this similarity, and the importance of Th1 cells for protection against intracellular protozoan parasites, we aimed to characterise the role of group 1 ILC s during Plasmodium infection. Methods We quantified group 1 ILC s in peripheral blood collected from subjects infected with with Plasmodium falciparum 3D7 as part of a controlled human malaria infection study, and in the liver and spleens of Pc AS ‐infected mice. We used genetically‐modified mouse models, as well as cell‐depletion methods in mice to characterise the role of group 1 ILC s during Pc AS infection. Results In a controlled human malaria infection study, we found that the frequencies of circulating ILC 1s and NK cells decreased as infection progressed but recovered after volunteers were treated with antiparasitic drug. A similar observation was made for liver and splenic ILC 1s in P. chabaudi chabaudi AS ( Pc AS )‐infected mice. The decrease in mouse liver ILC 1 frequencies was associated with increased apoptosis. We also identified a population of cells within the liver and spleen that expressed both ILC 1 and NK cell markers, indicative of plasticity between these two cell lineages. Studies using genetic and cell‐depletion approaches indicated that group 1 ILC s have a limited role in antiparasitic immunity during Pc AS infection in mice. Discussion Our results are consistent with a previous study indicating a limited role for natural killer ( NK ) cells during Plasmodium chabaudi infection in mice. Additionally, a recent study reported the redundancy of ILC s in humans with competent B and T cells. Nonetheless, our results do not rule out a role for group 1 ILC s in human malaria in endemic settings given that blood stage infection was initiated intravenously in our experimental models, and thus bypassed the liver stage of infection, which may influence the immune response during the blood stage. Conclusion Our results show that ILC 1s are lost early during mouse and human malaria, and this observation may help to explain the limited role for these cells in controlling blood stage infection.
Type of Medium:
Online Resource
ISSN:
2050-0068
,
2050-0068
DOI:
10.1002/cti2.2018.7.issue-1
Language:
English
Publisher:
Wiley
Publication Date:
2018
detail.hit.zdb_id:
2694482-0
