In:
Chemistry – A European Journal, Wiley, Vol. 28, No. 58 ( 2022-10-18)
Abstract:
S ‐Nitrosylation has been found to play an important role in regulating mitochondrial function. However, probes for detection of protein S ‐nitrosylation in mitochondria remain unexplored. Herein, a novel 4‐(pyridin‐4‐yl)vinyl‐substituted indole was designed, exhibiting a long‐wavelength emission and a high fluorescent quantum yield. Functionalization of the 7‐position of the indole ring with an arylphosphine ester resulted with probes with efficient mitochondria‐targeting ability. Furthermore, the indole‐arylphosphine displayed a significant fluorescence enhancement upon exposure to S ‐nitrosoglutathione (GSNO) at low micromolar concentrations in A431 cells. Taken together, this study provides a new indole‐based fluorescent probe with a unique long‐wavelength emission for direct detection of S ‐nitrosylation in mitochondria, which may represent a powerful tool for understanding the critical roles of S ‐nitrosylation within mitochondria of living organisms.
Type of Medium:
Online Resource
ISSN:
0947-6539
,
1521-3765
DOI:
10.1002/chem.202201494
Language:
English
Publisher:
Wiley
Publication Date:
2022
detail.hit.zdb_id:
1478547-X
