In:
Chemistry – A European Journal, Wiley, Vol. 25, No. 7 ( 2019-02), p. 1701-1705
Abstract:
Plant microRNAs (miRNAs) are naturally 2′‐ O ‐methylated at the 3′‐terminal; as a consequence, they cannot be efficiently detected by traditional target‐triggered polymerization reactions. Here, a simple but robust enzyme‐free sensing strategy was developed for plant miRNA analysis by using dynamic light scattering (DLS) to monitor the crosslinking of gold nanoparticles (AuNPs) amplified by click chemical ligation. Combining the enzyme‐free cycling chemical‐ligation‐mediated signal amplification, and the intrinsic outstanding ability of DLS for discriminating the extremely low level of particle aggregation in a large pool of monodisperse AuNPs, high sensitivity was achieved and as low as 78.6 f m plant miRNA could be easily detected.
Type of Medium:
Online Resource
ISSN:
0947-6539
,
1521-3765
DOI:
10.1002/chem.201805502
Language:
English
Publisher:
Wiley
Publication Date:
2019
detail.hit.zdb_id:
1478547-X
detail.hit.zdb_id:
1231884-X