In:
ChemBioChem, Wiley, Vol. 17, No. 10 ( 2016-05-17), p. 962-968
Abstract:
Hydrogen sulfide (H 2 S) is an important endogenous signaling molecule with multiple biological functions. New selective fluorescent turn‐on probes based on fast thiolyling of NBD (7‐nitro‐1,2,3‐benzoxadiazole) amine were explored for sensing H 2 S in aqueous buffer and in living cells. The syntheses of both probes are simple and quite straightforward. The probes are highly sensitive and selective toward H 2 S over other biologically relevant species. The fluorescein‐NBD‐based probe showed 65‐fold green fluorescent increase upon H 2 S activation. The rhodamine‐NBD‐based probe reacted rapidly with H 2 S ( t 1/2 ≈1 min) to give a 4.5‐fold increase in red fluorescence. Moreover, both probes were successfully used for monitoring H 2 S in living cells and in mice. Based on such probe‐based tools, we could observe H 2 O 2 ‐induced H 2 S biogenesis in a concentration‐dependent and time‐dependent fashion in living cells.
Type of Medium:
Online Resource
ISSN:
1439-4227
,
1439-7633
DOI:
10.1002/cbic.201600060
Language:
English
Publisher:
Wiley
Publication Date:
2016
detail.hit.zdb_id:
2020469-3
SSG:
12