In:
Cancer Medicine, Wiley, Vol. 6, No. 12 ( 2017-12), p. 2966-2971
Abstract:
Despite all the knowledge already gathered, the picture of somatic genetic changes in colorectal tumorigenesis is far from complete. Recently, germline and somatic mutations in the exonuclease domain of polymerase epsilon, catalytic subunit ( POLE ) gene have been reported in a small subset of microsatellite‐stable and hypermutated colorectal carcinomas ( CRC s), affecting the proofreading activity of the enzyme and leading to misincorporation of bases during DNA replication. To evaluate the role of POLE mutations in colorectal carcinogenesis, namely in advanced CRC , we searched for somatic mutations by Sanger sequencing in tumor DNA samples from 307 cases. Microsatellite instability and mutation analyses of a panel of oncogenes were performed in the tumors harboring POLE mutations. Three heterozygous mutations were found in two tumors, the c.857C 〉 G, p.Pro286Arg, the c.901G 〉 A, p.Asp301Asn, and the c.1376C 〉 T, p.Ser459Phe. Of the POLE ‐mutated CRC s, one tumor was microsatellite‐stable and the other had low microsatellite instability, whereas KRAS and PIK 3 CA mutations were found in one tumor each. We conclude that POLE somatic mutations exist but are rare in advanced CRC , with further larger studies being necessary to evaluate its biological and clinical implications.
Type of Medium:
Online Resource
ISSN:
2045-7634
,
2045-7634
DOI:
10.1002/cam4.2017.6.issue-12
Language:
English
Publisher:
Wiley
Publication Date:
2017
detail.hit.zdb_id:
2659751-2
