In:
Biotechnology and Bioengineering, Wiley, Vol. 45, No. 5 ( 1995-03-05), p. 379-386
Abstract:
Recycle batch fermentations using immobilized cells of Propionibacterium acidipropionici were studied for propionate production from whey permeate, de‐lactose whey permeate, and acid whey. Cells were immobilized in a spirally wound fibrous sheet packed in a 0.5‐L column reactor, which was connected to a 5‐L stirred tank batch fermentor with recirculation. The immobilized cells bioreactor served as a breeder for these recycle batch fermentations. High fermentation rates and conversions were obtained with these whey media without nutrient supplementation. It took ∼55 h to ferment whey permeate containing ∼45 g/L lactose to ∼20 g/L propionic acid. Higher propionate concentrations can be produced with various concentrated whey media containing more lactose. The highest propionic acid concentration obtained with the recycle batch reactor was 65 g/L, which is much higher than the normal maximum concentration of 35 to 45 g/L reported in the literature. The volumetric productivity ranged from 0.22 g/L · h to 0.47 g/L · h, depending on the propionate concentration and whey medium used. The corresponding specific cell productivity was 0.033 to 0.07 g/L · g cell. The productivity increased to 0.68 g/L · h when whey permeate was supplemented with 1% (w/v) yeast extract. Compared with conventional batch fermentation, the recycle batch fermentation with the immobilized cell bioreactor allows faster fermentation, produces a higher concentration of product, and can be run continually without significant downtime. The process also produced similar fermentation results with nonsterile whey media. © 1995 John Wiley & Sons, Inc.
Type of Medium:
Online Resource
ISSN:
0006-3592
,
1097-0290
DOI:
10.1002/bit.260450502
Language:
English
Publisher:
Wiley
Publication Date:
1995
detail.hit.zdb_id:
1480809-2
detail.hit.zdb_id:
280318-5
SSG:
12
