In:
Angewandte Chemie, Wiley, Vol. 127, No. 43 ( 2015-10-19), p. 12986-12990
Abstract:
The cooperativity of ligand binding is central to biological regulation and new approaches are needed to quantify these allosteric relationships. Herein, we exploit a suite of mass spectrometry (MS) experiments to provide novel insights into homotropic Cu‐binding cooperativity, gas‐phase stabilities and conformational ensembles of the D 2 ‐symmetric, homotetrameric copper‐sensitive operon repressor (CsoR) as a function of Cu I ligation state. Cu I binding is overall positively cooperative, but is characterized by distinct ligation state‐specific cooperativities. Structural transitions occur upon binding the first and fourth Cu I , with the latter occurring with significantly higher cooperativity than previous steps; this results in the formation of a holo‐tetramer that is markedly more resistant than apo‐, and partially ligated CsoR tetramers toward surface‐induced dissociation (SID).
Type of Medium:
Online Resource
ISSN:
0044-8249
,
1521-3757
DOI:
10.1002/ange.v127.43
DOI:
10.1002/ange.201506349
Language:
German
Publisher:
Wiley
Publication Date:
2015
detail.hit.zdb_id:
506609-8
detail.hit.zdb_id:
1479266-7