Infantile restrictive cardiomyopathy: cTnI-R170G/W impair the interplay of sarcomeric proteins and the integrity of thin filaments
Fig 2
Interactions of cTn with actin, tropomyosin and reconstituted thin filaments.
Binding kinetics of cTn containing cTnI-R170G/W (gray or white, respectively) or wildtype (WT, black) to immobilized (A) actin and (B) tropomyosin measured by SPR. Dissociation constants are given as KD ±SEM (n = 3). (C) Binding of troponin containing cTnI-variants to F-actin decorated with tropomyosin analyzed by cosedimentation. Data given as amount of bound cTnI relative to total cTnI in the sample ±SEM (n = 20). * indicates statistical significance with P<0.05; *** indicates statistical significance with P<0.001.