Safety and vaccine-induced HIV-1 immune responses in healthy volunteers following a late MVA-B boost 4 years after the last immunization
Fig 4
Frequency, function and phenotype of VACV-specific CD8 T cell responses.
A: Percentage of responders with positive ICS responses against MVA infected cells at the different time points. The equality of proportion between groups was determined using the function prop.test. *p = 0.017. B: Functional profile of VACV-specific CD8 T cells. The quality of the VACV-specific CD8 T cell response is characterized by the proportion of cells making every possible combination of the measured cytokines: IFN-γ (I); IL-2 (2); TNF-α (T) and CD107a (C). Responses are grouped and colour coded on the basis of the number of functions. The bar charts show the mean values and interquartile ranges (IQR) and the pie charts show the average proportion of the VACV-specific CD8 T cell responses according to the functions at weeks 0, 2, 4 and 12. “+”distributions that are different from the earliest time point (W0) within each category at p<0.05 using Student's T test or “#” Wilcoxon signed rank test. C: Phenotype of VACV-specific CD8 T cells. The graphic represents the distribution of the VACV-specific CD8 T cells at any time point based on CCR7 expression in combination with CD45RA within the Naïve (CD45RA+ CCR7+), T central memory (TCM: CD45RA- CCR7+), T effector memory (TEM: CD45RA- CCR7-) or terminally differentiated T effector memory (TEMRA: CD45RA+ CCR7-) phenotypes. Statistical differences were determined using ANOVA test (using the linear model y ~ x_patient + x_cellType + epsilon) followed by Tukey's honest significant difference criterion. *p<0.05.