ANKS1B Interacts with the Cerebral Cavernous Malformation Protein-1 and Controls Endothelial Permeability but Not Sprouting Angiogenesis
Fig 1
Silencing of ANKS1B does not affect endothelial proliferation and migration.
(A) Representative Western blot for HUVEC protein lysates probed with anti-ANKS1B antibody after transfection with non-silencing control siRNA or two different single ANKS1B siRNAs. VCP served as protein loading control. (B) Transfection of HUVEC with two independent siRNA duplexes led to approximately 80% mRNA knockdown rates compared to non-silencing control siRNA. (C) BrdU incorporation into DNA was only slightly increased after by silencing ANKS1B expression. (D) ANKS1B knockdown did not alter the migration speed of HUVEC after wounding a monolayer. ***, p < 0.001, n = 3 experiments. Scale bar equals 100 μm. Bar graphs show mean values, error bars indicate SD.