Label-Free Protein-RNA Interactome Analysis Identifies Khsrp Signaling Downstream of the p38/Mk2 Kinase Complex as a Critical Modulator of Cell Cycle Progression
Fig 3
Murine embryonic fibroblasts arrest in G2 in response to etoposide treatment.
(A) Gene expression changes identified by RNA-Seq following 6h of treatment with 20μM etoposide were analyzed for enrichments in GO terms. Cell cycle, and specifically mitotic processes, emerged in the top 10 most significant GO terms. (B) Protein-protein interactions-based network expansion of RBPs showing differential protein-RNA interactions upon etoposide treatment identifies enrichments for cyclin-dependent processes. (C) Cell cycle analysis of untreated (black line) and etoposide-treated (gray) cells reveals an accumulation of cells with 4N DNA content and decreased staining of the mitotic marker pHH3.