Allelic Variations at Four Major Maturity E Genes and Transcriptional Abundance of the E1 Gene Are Associated with Flowering Time and Maturity of Soybean Cultivars
Figure 1
Genotyping methods for E1 to E4 loci.
A–C: Genotyping of the E1 gene. Fragment was amplified by primer pair of TI-Fw and TI-Rv (A), subsequently subjected to TaqI digestion (B) and HinfI (C). The triangle represents the e1-nl type (lacking of fragment). The arrows in B and in C are representing e1-as and e1-fs genotypes, respectively. D: Genotyping of the E2 gene, fragment was amplified with primer pair of SoyGI_dCAP_Dra_fw and SoyGI_dCAP_Dra_rv, after digestion of DraI, the e2 was cut into two fragments, while E2 genotype remained uncut (arrows). E–G: Genotyping of the E3 gene. E: bands with three sizes were generated using the mixed primers. Band specific for E3-Mi and e3-tr were indicated by arrow and star, respectively. A 557 bp fragment (triangle) could be amplified either for E3-Ha or e3-Mo genotypes. F: PCR product yielded from primer pair E3_08094FW and E3_08417RV were digested with MseI. The E3-Ha genotype remained uncut, while e3-Mo allele (arrow) could be cut into 223 bp and 101 bp. G: specific determination of E3-fs type (arrow). CAPE primer pair E3-fsFW/E3-fsRV, restriction enzyme: AleI. H-J: Genotyping of the E4 gene. H: Using three mixed primers, a 837 fragment was specifically amplified from the E4 allele (arrow). I: CAPE primer pair e4-kam specific for e4-kam gene (arrow), enzyme AflII. J: CAPE primer pair e4-kes specific for e4-kes genotype (arrow), enzyme BspHI.