Functional Real-Time Optoacoustic Imaging of Middle Cerebral Artery Occlusion in Mice
Figure 2
Whole-brain optoacoustic images of a mouse.
A: Structural features visible in the cryosection (right) are matched with the corresponding optoacoustic image features (left). The representative stained cryosection shows the infarct area caused by 1 h MCAO followed by 24 h reperfusion. In the optoacoustic image, the area corresponding to the brain is enclosed by a green dotted line. The area affected by MCAO has been enclosed by a yellow dotted line. B: the multispectral data unmixed for the oxygenated and deoxygenated hemoglobin are overlaid on the single wavelength image from above (790 nm) in blue and red, respectively; the sagittal sinus is highly oxygenated due to the oxygen-isoflurane anesthetic. C: A coronal-slice set from a representative animal (distance between slices: 1 mm) with the multispectral unmixed data for deoxygenated hemoglobin in the brain presented in blue. The post ischemia set shows clear asymmetry (green arrow), while symmetry is intact in the pre ischemia set. Red and green squares in A and C indicate the ROIs for the ratio analysis.