PI3K p110δ Is Expressed by gp38−CD31+ and gp38+CD31+ Spleen Stromal Cells and Regulates Their CCL19, CCL21, and LTβR mRNA Levels
Figure 1
Immunofluorescence analysis of immune cell distribution and white pulp area.
Frozen sections of spleen and LN from p110δWT/WT, p110δD910A/D910A, and reconstituted mice were immunofluorescence-stained to detect T cells (CD3+, Thy1.2+), B cells (B220+), MMM (MOMA+) and DC (CD11c+). Representative images of spleen (A) and LN (B) sections for all conditions are shown (n = 6 mice/condition). Bar = 200 µm. (C) Measurement of white pulp area in hematoxylin/eosin-stained frozen spleen sections (3 sections/mouse, 6 mice/condition), quantified with ImageJ software. Mean ± SD; Kolmogorov-Smirnov test, ***p<0.001.