The Antarctic Krill Euphausia superba Shows Diurnal Cycles of Transcription under Natural Conditions
Figure 4
Phylogenetic relationships of E.superba opsins.
A) Alignment of the amino acid sequences of E.superba Rh1a and Rh1b opsins obtained by ClustalW. Seven transmembrane domains were predicted using TMPred (http://www.ch.embnet.org/software/TMPRED_form.html), indicated by lines above the sequence and labelled with Roman numerals. Asterisks (*) indicate amino acids functionally related to the long/middle wavelength-sensitive opsins, a colon (:) indicates the attachment site for the retinal Shiff's base linkage to the retinal chromophore. Sequences are colored according to the residue type. An alignment gap is indicated by a dash (–). B) Phylogenetic relationships of the EsRh1 (Rh1a and Rh1b) and opsin orthologs were obtained by neighbor-joining analysis. Bootstrap confidence values are based on 1,000 replicates. Scale bars indicate amino acid substitutions per site. LWS, long-wavelength sensitive; MWS, middle-wavelength sensitive; SWS, short-wavelength sensitive. Accession numbers: Litopenaeus vannamei: ABH00987; Neomysis Americana: ABI48886; Neogonodactylus oerstedii: ACU00212; Archaeomysis grebnitzkii: ABI48867; Euphausia superba LWS: ABI48874; Daphnia pulex: EFX76309; Uca pugilator Rh2: ADQ01810; Hemigrapsus sanguineus: Q25158; Charybdis japonica: AEL33282; Uca vomeris: ACT31580; Uca pugilator Rh1: ADQ01809; Uca pugilator Rh3: E5G6F2; Bos taurus: NP001014890.