Analysis of the Fibroblast Growth Factor System Reveals Alterations in a Mouse Model of Spinal Muscular Atrophy
Figure 1
Expression and regulation of FGFs in muscle and C2C12-cells.
(A) Transcript abundances relative to GAPDH in control muscle and scrambled siRNA transfected C2C12-cells. FGF-mRNA levels were measured by qRT-PCR in pooled samples of P5 control mice tissues and pooled samples of scrambled siRNA transfected cells relative to GAPDH as internal control. Transcript abundances were calculated from ΔCT-values. Unpaired t-tests of technical repetitions of measurements in cell culture compared to tissue abundances were performed (n = 2; n.s. = non significant, *p<0.05, **p<0.01, ***p<0.001). Bars represent means with standard deviations (SD). (B) Fold-change of FGF transcript levels in SMA-mice muscle and C2C12 cells after SMN-knockdown. FGF transcript concentration was measured by qRT-PCR in SMA-mice muscle and SMN-siRNA treated C2C12-cells. Transcript levels of SMA-mice and control animals were measured at P1 and P5. C2C12-cells were either transfected with SMN- or control scrambled-siRNA in 4 independent experiments (n = 4) with three replicates in each group. The knockdown for each experiment was monitored by western-blot analysis (Fig. S1). The fold-changes were calculated against each corresponding control group. Fold-changes of SMA-mice spinal cords were calculated against transcript levels of control mice of the same age. Results of SMN siRNA treated cells were compared to scrambled RNA transfected cells of the same experiment. SMA-mice transcript levels were tested against control animals by a Mann-Whitney test (n≥5, * p<0.05, ** p<0.01). mRNA-levels of SMN siRNA transfected cells were compared to scrambled siRNA transfected cells by a repeated measurements two way ANOVA (n = 4). Bars for fold changes represent means with standard error of mean (SEM).