Humanization and Characterization of an Anti-Human TNF-α Murine Monoclonal Antibody
Figure 1
Molecular model of the m357 variable regions.
The three-dimensional structure of m357 was generated by homology modeling using the crystal structures of the anti-breast tumor antibody SM3 (PDB code 1SM3) and the anti-Thermus aquaticus DNA polymerase I antibody (PDB code 1AY1) for the VH and VL domains, respectively. The six residues which are preferably mutated to “human” amino acid are indicated by stick structures (green). Four residues in the VH framework and two residues in the VL framework were humanized. Valine 59, which is within about 5 Å of the CDR2 in light chain and may affect the antigen binding, is retained for preserving the antigen binding affinity and shown as stick structure (orange). The CDR loops are shown in red color.