MMP19 Is Essential for T Cell Development and T Cell-Mediated Cutaneous Immune Responses
Figure 3
MMP19−/− mice exhibit reduced T cell activation in CHS.
Using flow cytometry cells of inguinal (A and B) or draining lymph nodes (C and D) from wild-type (+/+) and MMP19-deficient (−/−) mice were analyzed for the indicated activation markers, all gated on CD8+ T cells. (A) Unsensitized MMP19−/− mice show slight decrease of T cells positive for CD62L and CD95/CD25. (B) MMP19−/− mice analyzed 24 h after abdominal FITC painting (sensitization) exhibit higher numbers of naive T cells (CD62L+) and decreased numbers of activated T cells compared to MMP19+/+ mice. (C) In CHS reaction 48 h after FITC challenge on ears, draining lymph nodes of MMP19−/− mice show significantly reduced numbers of cells positive for activation and memory markers. (D) Reduced numbers of CD25+ and high numbers of CD62L+ cells are still present in MMP19−/− mice after 72 h while other activation markers were comparable to those of MMP19+/+ mice. In A and B two scales are used to match relevant data from an identical experiment. Significant values (p<0.05) are marked by asterisk. Each analysis was carried out four times with MMP19+/+ (n = 4) and MMP19−/− mice (n = 4) per experiment.