Serodiagnostic antigens of Clonorchis sinensis identified and evaluated by high-throughput proteogenomics
Fig 3
Recombinant antigenic proteins produced and purified in large scale using wheat germ cell-free protein synthesis system (WGCFS).
The recombinant proteins were expressed using WGCFS, purified, and deployed on SDS-PAGE gel. The arrow indicates a purified recombinant protein having expected molecular mass. M, protein size marker; T, total translation mix; F, flow-through; N, non-reducing elution; R, reducing elution; r, resin after eluted.