Serodiagnostic antigens of Clonorchis sinensis identified and evaluated by high-throughput proteogenomics
Fig 1
Antigenicity of recombinant proteins on protein array chips and selected candidates.
(A) Fluorescent images of the recombinant proteins reacting to clonorchiasis patients’ sera and normal controls’ sera on protein array chips. The spots in boxes are positive and negative control proteins. Two spots top left are bovine serum albumin and wheat-germ extract itself, included as negative controls. Another 8 spots, top left to down, are recombinant C. sinensis 26 kDa glutathione-S-transferase (Cs26GST), Cs28GST, phosphoglycerate kinase, cathepsin F, and WGCFS translates of Cs26GST-1, Cs26GST-2, Cs28GST-1 and Cs28GST-2, included as positive controls. (B) Plot of fluorescence intensity (FI) of the recombinant proteins. Eighteen antigenic candidate proteins showing a higher FI were selected (above red line). Data are expressed as mean ± SD of triplicate measurements.