Sodium-bile acid co-transporter is crucial for survival of a carcinogenic liver fluke Clonorchis sinensis in the bile
Fig 7
Effect of CsSBAT silencing using RNAi.
(A) Downregulation of CsSBAT transcripts measured using RT-qPCR. No dsRNA, flukes incubated without dsRNA; dsRNA1 and dsRNA2, flukes incubated in CsSBAT-dsRNA1 or CsSBAT-dsRNA2. (B) Survival and (C) motility of Clonorchis sinensis adults incubated in CsSBAT-dsRNA1 or CsSBAT-dsRNA2 plus 0.075% bile solution. In control groups, C. sinensis were incubated in 1× Locke’s solution or plus 0.075% bile solution. Data represent mean ± standard error. †p < 0.05, ‡p < 0.01, compared to flukes in the bile only.