Immunostaining of Ectocarpus Cells
- Susana M. Coelho1,2,4,
- Delphine Scornet1,2,
- Sylvie Rousvoal1,2,
- Nick Peters1,2,
- Laurence Dartevelle1,2,
- Akira F. Peters2,3 and
- J. Mark Cock1,2
- 1UPMC Université Paris 06, The Marine Plants and Biomolecules Laboratory, UMR 7139, Station Biologique de Roscoff, 29682 Roscoff Cedex, France
- 2CNRS, UMR 7139, Laboratoire International Associé Dispersal and Adaptation in Marine Species, Station Biologique de Roscoff, 29682 Roscoff Cedex, France
- 3Bezhin Rosko, 29250 Santec, France
Abstract
This article describes an immunostaining protocol for Ectocarpus that was optimized for the detection of tubulin but could be used with any suitable antibody. Ectocarpus has small but relatively transparent cells and the uniseriate filaments can be grown directly attached to the surface of microscope slides. These features make Ectocarpus particularly suitable for high resolution imaging approaches, both in vivo or after fixation. All incubations described below are carried out on a platform shaker at room temperature. Use high-quality microscope slides to avoid imperfections in the glass that can be a problem for confocal laserscan microscopy analysis.
Footnotes
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↵4 Correspondence: coelho{at}sb-roscoff.fr
- © 2012 Cold Spring Harbor Laboratory Press
