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The occurrence of toxic cyanobacterium Cylindrospermopsis raciborskii and its toxin cylindrospermopsin in the Huong River, Thua Thien Hue province, Vietnam

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Abstract

This research reports the presence of species Cylindrospermopsis raciborskii and cylindrospermopsin (CYN) in the Huong River and the relationship between species with environmental factors to find a scientific basis for predicting the risk of pollution of the species and CYN in waters. Strains of C. raciborskii isolated from the river were also identified as potentially toxin-producing through the determination of the presence of toxins in the cultures by ELISA; the presence of the genes involved by PCR confirms the CYN-producing ability of species C. raciborskii from this water body. Our results have confirmed the presence of toxic cyanobacteria C. raciborskii in the Huong River. C. raciborskii from the Huong River are mostly solitary, straight trichomes. Analyses of all C. raciborskii strains from the Huong River by ELISA for cylindrospermopsin were positive. The contents of cylindrospermopsin (CYN) in each strain were different, ranging from 5.25 ng mg−1 wet weight in CR1DD to 70.83 ng mg−1 wet weight in CR1NY. PCR analysis confirmed that the genes involved in the production of this cyanotoxin were present in C. raciborskii. The relationship between densities and toxicity showed a correlation coefficient R of 0.88. This was a relatively high positive correlation index, indicating the close links between densities and toxins: toxin CYN concentrations increased when C. raciborskii densities increased.

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Acknowledgements

This work was supported by the Vietnamese National Foundation for Science and Technology Development (NAFOSTED) grant 196.06.73.09. We appreciate the effort of an anonymous reviewer and the useful comments and suggestions for improving the manuscript.

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Authors and Affiliations

  1. Institute of Biotechnology, Hue University, Road 10, Phu Thuong commune, Phu Vang district, Thua Thien Hue province, Vietnam

    Thi Thu Lien Nguyen & Tien Hien Hoang

  2. Department of Environmental Hydrobiology, Institute of Environmental Technology, Vietnam Academy of Science and Technology, A30, 18 Hoang Quoc Viet Street, Cau Giay district, Hanoi, Vietnam

    Trung Kien Nguyen & Thi Thuy Duong

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  1. Thi Thu Lien Nguyen
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  2. Tien Hien Hoang
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Correspondence to Thi Thu Lien Nguyen or Thi Thuy Duong.

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Nguyen, T.T.L., Hoang, T.H., Nguyen, T.K. et al. The occurrence of toxic cyanobacterium Cylindrospermopsis raciborskii and its toxin cylindrospermopsin in the Huong River, Thua Thien Hue province, Vietnam. Environ Monit Assess 189, 490 (2017). https://doi.org/10.1007/s10661-017-6209-7

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