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Alkalibacillus aidingensis sp. nov., an Bacterium Isolated from Aiding Lake in Xinjiang Province, North-West China

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Abstract

A bacterial strain, Gram staining negative, aerobic, long rod, motile bacterium with flagellum, designated strain YIM 98829T, was isolated from the Aiding Lake in Xinjiang province, North-West China. The isolate produced oval subterminal endospores in swollen sporangia. The predominant menaquinone was MK-7. The cell wall peptidoglycan contained ornithine, serine, aspartic acid, glutamic acid, and alanine, while diaminopimelic acid could not be detected. The major whole-cell sugars contained xylose, glucose, galactose, and mannose. Diphosphatidylglycerol, phosphatidylglycerol, one unknown phospholipid, and two unidentified aminophospholipids were part of the polar lipid profile. Iso-C15:0 and anteiso-C15:0 were the major fatty acids. The DNA G + C content of the type strain was 38.0 mol%. Phylogenetic analysis indicated that the isolate belongs to the genus Alkalibacillus. However, it differed from its closest relatives, A. haloalkaliphilus DSM 5271T (97.04%), A. filiformis 4AGT (96.99%), and A. silvisoli BM2T (96.95%) in some physiological characteristics. DNA–DNA hybridization result indicated low levels of relatedness between strain YIM 98829T and A. haloalkaliphilus JCM 12303T (16.9%). On the basis of physiological, phenotypic, and chemotaxonomic data, strain YIM 98829T represents a novel species of genus Alkalibacillus, for which the name Alkalibacillus aidingensis sp. nov. is proposed. The type strain is YIM 98829T (= NBRC 114103T = CGMCC 1.17260T = DSM 112470T).

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References

  1. Jeon CO, Lim JM, Lee JM, Xu LH, Jiang CL, Kim CJ (2005) Reclassification of Bacillus haloalkaliphilus Fritze 1996 as Alkalibacillus haloalkaliphilus gen. nov., comb. Nov. and the description of Alkalibacillus salilacus sp. nov., a novel halophilic bacterium isolated from a salt lake in China. Int J Syst Evol Microbiol 55(5):1891–1896

    Article  CAS  Google Scholar 

  2. Fritze D (1996) Bacillus haloalkaliphilus sp. nov. Int J Syst Bacteriol 46:98–101

    Article  Google Scholar 

  3. Romano I, Lama L, Nicolaus B, Gambacorta A (2005) Alkalibacillus filiformis sp. nov., isolated from a mineral pool in Campania. Italy. Int J Syst Evol Microbiol 55(6):2395–2399

    Article  CAS  Google Scholar 

  4. Usami R, Echigo A, Fukushima T, Mizuki T, Yoshida Y, Kamekura M (2007) Alkalibacillus silvisoli sp. nov., an alkaliphilic moderate halophile isolated from non-saline forest soil in Japan. Int J Syst Evol Microbiol 57(4):770–774

    Article  CAS  Google Scholar 

  5. Tian XP, Dastager SG, Lee JC, Tang SK, Zhang YQ, Park DJ, Kim CJ, Li WJ (2007) Alkalibacillus halophilus sp. nov., a new halophilic species isolated from hypersaline soil in Xin-Jiang province, China. Syst Appl Microbiol 30(4):268–272

    Article  CAS  Google Scholar 

  6. Yoon JH, Kang SJ, Jung YT, Lee MH, Oh TK (2010) Alkalibacillus flavidus sp. nov., isolated from a marine solar saltern. Int J Syst Evol Microbiol 60(2):434–438

    Article  CAS  Google Scholar 

  7. Pérez-Davó A, Aguilera M, Ramos-Cormenzana A, Monteoliva-Sánchez M (2014) Alkalibacillus almallahensis sp. nov., a halophilic bacterium isolated from an inland solar saltern. Int J Syst Evol Microbiol 64(Pt6):2066–2071

    Article  Google Scholar 

  8. Nielsen P, Fritze D, Priest FG (1995) Phenetic diversity of alkaliphilic Bacillus strains: proposal for nine new species. Microbiology 141:1745–1761

    Article  CAS  Google Scholar 

  9. Murray RG, Doetsch RN, Robinow CF (1994) Determinative and cytological light microscopy. In: Gerhardt P, Murray RG, Wood WA, Krieg NR (eds) Methods for general and molecular bacteriology. American Society for Microbiology, Washington, pp 21–41

    Google Scholar 

  10. Baron EJ, Finegold SM (1990) Bailey and scott’s diagnostic microbiology, 8th edn. Mosby, St. Louis

    Google Scholar 

  11. Xu P, Li WJ, Tang SK, Zhang YQ, Chen GZ, Chen HH, Xu LH, Jiang CL (2005) Naxibacter alkalitolerans gen. nov., sp. nov., a novel member of the family ‘Oxalobacteraceae’ isolated from China. Int J Syst Evol Microbiol 55:1149–1153

    Article  CAS  Google Scholar 

  12. Smibert R, Krieg NRM (1994) Phenotypic characterization. In: Gerhardt P, Murray RG, Wood WA, Krieg NR (eds) Methods for general and molecular bacteriology. American Society for Microbiology, Washington, pp 607–654

    Google Scholar 

  13. Harrigan WF, McCance ME (1976) Laboratory methods in food and dairy microbiology. Academic Press, London

    Google Scholar 

  14. Amoozegar MA, Bagheri M, Makhdoumi-Kakhki A, Didari M, Schumann P, Spröer C, Sánchez-Porro C, Ventosa A (2014) Oceanobacilluslimi sp. nov., a moderately halophilic bacterium from a salt lake. Int J Syst Evol Microbiol 64:1284–1289

    Article  CAS  Google Scholar 

  15. Yang L, Huang HW, Wang Y, Kou YR, Yin M, Li Y, Wang XQ, Zhao GF, Zhu WY, Tang SK (2020) Paenibacillus turpanensis sp. nov., isolated from a salt lake of Turpan city in Xinjiang province, north-west China. Arch Microbiol. https://doi.org/10.1007/s00203-020-02003-w

    Article  PubMed  Google Scholar 

  16. Li WJ, Xu P, Schumann P, Zhang YQ, Pukall R, Xu LH, Stackebrandt E, Jiang CL (2007) Georgenia ruanii sp. nov., a novel actinobacterium isolated from forest soil in Yunnan (China), and emended description of the genus Georgenia. Int J Syst Evol Microbiol 57:1424–1428

    Article  Google Scholar 

  17. Boratyn GM, Camacho C, Cooper PS, Coulouris G, Fong A, Ma N, Madden TL, Matten WT, McGinnis SD, Merezhuk Y, Raytselis Y, Sayers EW, Tao T, Ye J, Zaretskaya I (2013) BLAST: a more efficient report with usability improvements. Nucleic Acids Res W29–33

  18. Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG (1997) The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882

    Article  CAS  Google Scholar 

  19. Saitou N, Nei M (1987) The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425

    CAS  Google Scholar 

  20. Fitch WM (1971) Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20:406–416

    Article  Google Scholar 

  21. Felsenstein J (1981) Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17:368–376

    Article  CAS  Google Scholar 

  22. Kumar S, Stecher G, Tamura K (2016) MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol 33:1870–1874

    Article  CAS  Google Scholar 

  23. Felsenstein J (1985) Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791

    Article  Google Scholar 

  24. Meier-Kolthoff JP, Auch AF, Klenk HP, Göker M (2013) Genome sequence-based species delimitation with confidence intervals and improved distance functions. BMC Bioinform 14:60

    Article  Google Scholar 

  25. Richter M, Ramon RM, Oliver GF, Peplies J (2016) JSpeciesWS: a web server for prokaryotic species circumscription based on pairwise genome comparison. Bioinform 32(6):929–931

    Article  CAS  Google Scholar 

  26. Stamatakis A (2014) RAxML version 8: a tool for phylogenetic analysis and post-analysis of large phylogenies. Bioinformatics 30:1312–1313

    Article  CAS  Google Scholar 

  27. Stamatakis A, Hoover P, Rougemont J (2008) A rapid bootstrap algorithm for the RAxML web servers. Syst Biol 57:758–771

    Article  Google Scholar 

  28. Schumann P (2011) Peptidoglycan structure. Methods Microbiol 38:101–129

    Article  CAS  Google Scholar 

  29. Schumann P, Kalensee F, Cao J, Criscuolo A, Clermont D, Köhler JM, Meier-Kolthoff JP, Neumann-Schaal M, Tindall BJ, Pukall R (2021) Reclassification of Haloactinobacterium glacieicola as Occultella glacieicola gen. nov., comb. nov., of Haloactinobacterium album as Ruania alba comb. nov, with an emended description of the genus Ruania, recognition that the genus names Haloactinobacterium and Ruania are heterotypic synonyms and description of Occultella aeris sp. nov., a halotolerant isolate from surface soil sampled at an ancient copper smelter. Int J Syst Evol Microbiol 71(4):004769

    Article  CAS  Google Scholar 

  30. Hasegawa T, Takizawa M, Tanida S (1983) A rapid analysis for chemical grouping of aerobic actinomycetes. J Gen Appl Microbiol 29(4):319–322

    Article  CAS  Google Scholar 

  31. Collins MD, Jones D (1980) Lipids in the classification and identification of coryneform bacteria containing peptidoglycans based on 2,4-diaminobutyric acid. J Appl Bacteriol 48:459–470

    Article  CAS  Google Scholar 

  32. Minnikin DE, Collins MD, Goodfellow M (1979) Fatty acid and polar lipid composition in the classification of Cellulomonas, Oerskovia and related taxa. J Appl Bacteriol 47:87–95

    Article  CAS  Google Scholar 

  33. Collins MD, Pirouz T, Goodfellow M, Minnikin DE (1977) Distribution of menaquinones in actinomycetes and corynebacteria. J Gen Microbiol 100:221–230

    Article  CAS  Google Scholar 

  34. Tamaoka J, Katayama-Fujimura Y, Kuraishi H (1983) Analysis of bacterial menaquinone mixtures by high performance liquid chromatography. J Appl Bacteriol 54:31–36

    Article  CAS  Google Scholar 

  35. Wayne LG, Brenner DJ, Colwell RR, Grimont PAD, Kandler O, Krichevsky MI, Moore LH, Moore WEC, Murray RGE, Stackebrandt E, Starr MP, Truper HG (1987) International committee on systematic bacteriology: announcement report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464

    Article  Google Scholar 

Download references

Funding

Funding was provided by National Natural Science Foundation of China (Grant nos. 31760003, 31270055, 31500011), Major Science and Technology Projects of Yunnan Province (Development and application of digitalization of biological resources), Natural Science Foundation of Yunnan Province (Grant no. 2017FB039), South and Southeast Asia Cooperation Base on Microbiological Resource Prevention and Utilization (Grant no. 2018IA100), Major Science and Technology Projects of Yunnan Province (Digitalization, development and application of biotic resource) (Grant no. 202002AA100007).

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Author notes

  1. Rui Li and Li Yang have equally contributed to this work.

Authors and Affiliations

  1. Yunnan Institute of Microbiology, Key Laboratory for Conservation and Utilization of Bio-Resource, and Key Laboratory for Microbial Resources of the Ministry of Education, School of Life Sciences, Yunnan University, Kunming, 650091, People’s Republic of China

    Rui Li, Li Yang, Chang-Gai Mu, Yong-Jiang Shi, Wen-Yong Zhu & Shu-Kun Tang

  2. Yunnan Key Laboratory of Vaccine Research & Development on Severe Infections Diseases, Institute of Medical Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Kunming, 650118, People’s Republic of China

    Wen-Yong Zhu

  3. School of Medicine, Yunnan University, Kunming, 650091, People’s Republic of China

    Min Yin

  4. Life Science and Technology School, Lingnan Normal University, Zhanjiang, 524048, People’s Republic of China

    Yun Wang

  5. Urumqi Customs Technology Center, Urumqi, 830011, People’s Republic of China

    Gang-Qiang Jiang

  6. China General Microbiological Culture Collection Center, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, People’s Republic of China

    Yu-Guang Zhou & Man Cai

  7. Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures, Braunschweig, Germany

    Rüdiger Pukall & Meina Neumann-Schaal

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  1. Rui Li
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  2. Li Yang
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Correspondence to Wen-Yong Zhu or Shu-Kun Tang.

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The NCBI GenBank accession number for the 16S rRNA gene sequence of strain YIM 98829T is MN251019. The draft whole-genome sequence for YIM 98829T has been deposited at DDBJ/ENA/GenBank under Accession Number GCA_014595945.1.

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Li, R., Yang, L., Pukall, R. et al. Alkalibacillus aidingensis sp. nov., an Bacterium Isolated from Aiding Lake in Xinjiang Province, North-West China. Curr Microbiol 78, 3307–3312 (2021). https://doi.org/10.1007/s00284-021-02587-6

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