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Nachweis der Ausbildung übermolekularer Teilchen in Lösungen vonDNA durch Messung der Lichtstreuung und Viskosität

Studies on the formation of supermolecular particles in solutions of DNA by means of light scattering and rheological methods

  • Organische Chemie und Biochemie
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Abstract

Buffered solutions of various preparations of calves thymus and salmon sperm resp. were investigated by optical and rheological methods. The fine structure of measuredP(θ)-light scattering functions was interpreted as being caused by large supermolecular particles in solution. These could be analysed by use of theRayleigh-Gans-theory and were found to have low density and to be very sensitive against temperature and shear. Number and size of supermolecular particles inDNA-solutions increase steadily when the solutions are allowed to rest for some days.

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Authors and Affiliations

  1. Institut für Makromolekulare Chemie, Technische Hochschule Darmstadt, D-6100, Darmstadt, Bundesrepublik Deutschland

    Erich Gruber

  2. Sonderforschungsbereich 41 “Chemie und Physik der Makromoleküle”, Mainz-Darmstadt

    Rosel Gruber

  3. Institut für Physikalische Chemie, Universität Graz, A-8010, Graz, Österreich

    Josef Schurz

Authors
  1. Erich Gruber
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  2. Rosel Gruber
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  3. Josef Schurz
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Gruber, E., Gruber, R. & Schurz, J. Nachweis der Ausbildung übermolekularer Teilchen in Lösungen vonDNA durch Messung der Lichtstreuung und Viskosität. Monatshefte für Chemie 109, 1115–1137 (1978). https://doi.org/10.1007/BF00913014

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  • DOI: https://doi.org/10.1007/BF00913014

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