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The human vitronectin (complement S-protein) gene maps to the centromeric region of 17q

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Summary

Vitronectin (complement S-protein, serumspreading factor, epibolin) is a multifunctional glycoprotein that mediates cell-to-substrate adhesion, inhibits the cytolytic action of the terminal complement cascade in vitro and binds to several serine protease inhibitors of the serpin family, viz. antithrombin III, plasminogen activator inhibitor I (PAI-1) and II (PAI-2), heparin cofactor II and protease nexin. Using high resolution fluorescence in situ hybridization, we mapped the vitronectin gene to the centromeric region of the long arm of chromosome 17 corresponding to 17q11. The location was confirmed by co-hybridization with the centromerespecific alphoid probe p17H8 (D17Z1) and by chromosome banding with 4,6-diamidino-2-phenylindole-dihydrochloride (DAPI). None of the previously mapped genes that are evolutionary related to vitronectin are located on the same chromosome.

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References

  • Brigati DJ, Myerson D, Leary JJ, Spalholz B, Travis SZ, Fong CKY, Hsiung GD, Ward DC (1983) Detection of viral genomes in cultured cells and paraffin-embedded tissue sections using biotin-labeled hybridization probes. Virology 126:32–50

    Article  CAS  PubMed  Google Scholar 

  • Buckley MF, Loveland KA, McKinstry WJ, Garsom OM, Goding JW (1990) Plasma cell membrane glycoprotein PC-1. cDNA cloning of the human molecule, amino acid sequence, and chromosomal location. J Biol Chem 265:17506–17511

    CAS  PubMed  Google Scholar 

  • Cohen IH, Chan HS, Track RK, Kidd KK (1990) Book 5: Human maps. In: O'Brien SJ (ed) Genetic maps: locus maps of complex genomes. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY

    Google Scholar 

  • Ehrlich HJ, Gebbink RK, Keijer J, Linders M, Preissner KT, Pannekoek H (1990) Alteration of serpin specifity by a protein cofactor. Vitronectin endows plasminogen activator inhibitor 1 with thrombin properties. J Biol Chem 265:13029–13035

    CAS  PubMed  Google Scholar 

  • Huhtala P, Eddy RL, Fan YS, Byers BG, Shows TB, Traggvason K (1990) Completion of the primary structure of the human type IV collagenase preproenzyme and assignment of the gene (CLG4) to the q21 region of chromosome 16. Genomics 6:554–559

    Article  CAS  PubMed  Google Scholar 

  • Hunt LT, Barker HR, Chen HR (1987) A domain structure common to hemopexin, vitronectin, interstitial collagenase, and a collagenase homolog. Protein Seq Data Anal 1:21–26

    CAS  PubMed  Google Scholar 

  • Jenne DE, Stanley KK (1987) Nucleotide sequence and organization of the human S-protein gene: repeating peptide motifs in the “pexin” family and a model for their evolution. Biochemistry 26:6735–6742

    Article  CAS  PubMed  Google Scholar 

  • Kubota K, Hayashi M, Oishi N, Sakaki Y (1990) Polymorphism of the human vitronectin gene causes vitronectin blood type. Biochem Biophys Res Commun 167:1355–1360

    Article  CAS  PubMed  Google Scholar 

  • Langer PR, Waldrop AA, Ward DC (1981) Enzymatic synthesis of biotin-labeled polynucleotides: novel nucleic acid affinity probes. Proc Natl Acad Sci USA 78:6633–6637

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Law ML, Cai GY, Hartz JA, Jones C, Kao FT (1988) The hemopexin gene maps to the same location as the β-globin gene cluster on human chromosome 11. Genomics 3:48–52

    Article  CAS  PubMed  Google Scholar 

  • Lichter P, Cremer T (1991) Chromosome analysis by non-isotopic in situ hybridization. In: Rooney DE, Czepulkowski BH (eds) Human cytogenetics: a practical approach. IRL Press, Oxford (in press)

    Google Scholar 

  • Lichter P, Cremer T, Borden J, Manuelidis L, Ward DC (1988) Delineation of individual human chromosomes in metaphase and interphase cells by in situ suppression hybridization using recombinant DNA libraries. Hum Genet 80:224–234

    Article  CAS  PubMed  Google Scholar 

  • Lichter P, Tang CC, Call K, Hermanson G, Evans GA, Housman D, Ward DC (1990) High resolution mapping of human chromosome 11 by in situ hybridization with cosmid clones. Science 247:64–69

    Article  CAS  PubMed  Google Scholar 

  • Matrisian LM (1990) Metalloproteinases and their inhibitors in matrix remodeling. Trends Genet 6:121–125

    Article  CAS  PubMed  Google Scholar 

  • McGuire EA, Peacock ME, Inhorn RC, Siegel NR, Tollefsen DM (1988) Phosphorylation of vitronectin by protein kinase in human plasma. Identification of a unique phosphorylation site in the heparin-binding domain. J Biol Chem 263:1942–1945

    CAS  PubMed  Google Scholar 

  • Naylor SL, Altruda F, Marshall A, Silengo L, Bowman BH (1987) Hemopexin is located to human chromosome 11. Somat Cell Mol Genet 13:355–358

    Article  CAS  PubMed  Google Scholar 

  • Patthy L (1987) Detecting homology of distantly related proteins with consensus sequences. J Mol Biol 198:567–577

    Article  CAS  PubMed  Google Scholar 

  • Podack ER, Müller-Eberhardt HJ (1979) Isolation of human Sprotein, an inhibitor of the membrane attack complex of complement. J Biol Chem 254:9808–9814

    CAS  PubMed  Google Scholar 

  • Preissner KT (1991) The structure and biological role of vitronectin. Annu Rev Cell Biol 7:275–310

    Article  CAS  PubMed  Google Scholar 

  • Preissner KT, Jenne DE (1991) Vitronectin: a new molecular connection in haemostasis. Thromb Haemost (in press)

  • Ruoslahti E (1991) Integrins. J Clin Invest 87:1–5

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Sane DC, Moser TL, Parker CJ, Seiffert D, Loskutoff DJ, Greenberg CS (1990) Highly sulfated glycosaminoglycans augment the cross-linking of vitronectin by guinea pig liver transglutaminase. Functional studies of the cross-linked vitronectin multimers. J Biol Chem 265:3543–3548

    CAS  PubMed  Google Scholar 

  • Spurr NK, Gough Gosden J, Rout D, Porteous DJ, Docherty AJ (1988) Restriction fragment length polymorphism analysis and assignment of the metalloproteinase stromelysin and collagenase to the long arm of chromosome 11. Genomics 2:119–127

    Article  CAS  PubMed  Google Scholar 

  • Stanley KK (1986) Homology with hemopexin suggests a possible scavening function for S-protein/vitronectin. FEBS Lett 199:249–253

    Article  CAS  PubMed  Google Scholar 

  • Tollefsen DM, Weigel CJ, Kabeer MH (1990) The presence of methionine or threonine at position 381 in vitronectin is correlated with proteolytic cleavage at arginine 379. J Biol Chem 265:9778–9781

    CAS  PubMed  Google Scholar 

  • Tschopp J, Masson D, Schaefer S, Peitsch M, Preissner KT (1988) The heparin binding domain of S-protein/vitronectin binds to complement components C7, C8, and C9 and perforin from cytolytic T-cells and inhibits their lytic activities. Biochemistry 27:4103–4109

    Article  CAS  PubMed  Google Scholar 

  • Viskochill D, Buchberg AM, Xu G, Cawthon RM, Stevenson J, Wolff RK, Culver M, Carey JC, Copeland NG, Jenkins NA, White R, O'Connell P (1990) Deletions and a translocation interrupt a cloned gene at the neurofibromatosis type 1 locus. Cell 62:187–192

    Article  Google Scholar 

  • Wallace MR, Marchuk DA, Anderson LB, Letcher R, Odeh HM, Saulino AM, Fountain JW, Brereton A, Nicholson J, Mitchell AL, Brownstein BH, Collins FS (1990a) Type 1 neurofibromatosis gene: identification of a large transcript disrupted in three NF1 patients. Science 249:181–186

    Article  CAS  PubMed  Google Scholar 

  • Wallace RM, Anderson LB, Fountain JW, Odeh HM, Viskochil D, Marchuk DA, O'Connell P, White R, Collins FS (1990b) A chromosome jump crosses a translocation breakpoint in the von Recklinghausen neurofibromatosis region. Genes Chromosomes Cancer 2:271–277

    Article  CAS  PubMed  Google Scholar 

  • Waye J, Willard H (1986) Structure, organization and sequence of a alpha satellite DNA from human chromosome 17: evidence for evolution by unequal crossing-over and an ancestral pentamer repeat shared with the human X chromosome. Mol Cell Biol 6:3156–3165

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Yagle MK, Parruti G, Xu W, Ponder BAJ, Solomon E (1990) Genetic and physical mapping of the von Recklinghausen neurofibromatosis (NF1) region on chromosome 17. Proc Natl Acad Sci USA 87:7255–7259

    Article  CAS  PubMed  PubMed Central  Google Scholar 

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Authors and Affiliations

  1. Deutsches Krebsforschungszentrum, Im Neuenheimer Feld 280, W-6900, Heidelberg, Germany

    Thomas M. Fink & Peter Lichter

  2. Institute of Biochemistry, University of Lausanne, CH-1066, Epalinges, Switzerland

    Dieter E. Jenne

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  1. Thomas M. Fink
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  2. Dieter E. Jenne
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  3. Peter Lichter
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Fink, T.M., Jenne, D.E. & Lichter, P. The human vitronectin (complement S-protein) gene maps to the centromeric region of 17q. Hum Genet 88, 569–572 (1992). https://doi.org/10.1007/BF00219346

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  • DOI: https://doi.org/10.1007/BF00219346

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