Skip to main content
SpringerLink
Log in

Timing of the response of coleoptiles to the application and withdrawal of various auxins

  • Published:
Planta Aims and scope Submit manuscript

Summary

A study was made of the time courses of growth promotion and the reversal of growth promotion upon the addition and withdrawal of various auxins. Growth promotion by 1-naphthaleneacetic acid (NAA) and 2,4-dichlorophenoxyacetic acid (2,4-D) occurs more slowly and is less vigorous than growth promotion by the same concentration of indoleacetic acid (IAA).

The time required for the reversal of the stimulation of elongation by auxin is many times greater for 2,4-D-stimulated growth than for IAA- or NAA-stimulated growth (80 min vs. about 10 min). This difference appears to be due to the sluggish exit of 2,4-D since (1) experiments with labeled auxins show that 2,4-D moves out of the tissue more slowly than IAA, and (2) it is possible to shorten the time required for a decline in elongation rate after the removal of 2,4-D to 13 min by adding an auxin antagonist (p-chlorophenoxyisobutyric acid).

The rapid reversal of the hormonal stimulation of growth is discussed in relation to possible mechanisms of action of auxin.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Axelrod, B., and A. T. Jagendorf: The fate of phosphatase, invertase and peroxidase in autolysing leaves. Plant Physiol. 26, 406–410 (1951).

    Google Scholar 

  • Afridi, M. M. R. K., and E. Hewitt: The inducible formation and stability of nitrate reductase in higher plants. J. exp. Bot. 15, 251–271 (1964).

    Google Scholar 

  • Bonner, J.: The molecular biology of development. New York: Oxford Univ. Press 1965.

    Google Scholar 

  • —, and R. J. Foster: The growth-time relationships of the auxin-induced growth in Avena coleoptile sections. J. exp. Bot. 6, 293–302 (1955).

    Google Scholar 

  • Cleland, R.: Auxin-induced cell wall loosening in the presence of actinomycin-D. Plant Physiol. 40, 595–600 (1965).

    PubMed  Google Scholar 

  • Evans, M. L., and P. M. Ray: Timing of the auxin response in coleoptiles and its implications regarding auxin action. J. gen. Physiol., in press (1969).

  • Foster, R. J., D. H. McRae, and J. Bonner: Auxin-antiauxin interaction at high auxin concentrations. Plant Physiol. 30, 323–327 (1955).

    Google Scholar 

  • Glasziou, K. T., J. C. Waldron, and T. A. Bull: Control of invertase synthesis in sugar cane. Plant Physiol. 41, 282–288 (1966).

    Google Scholar 

  • Hertel, R., and R. Flory: Auxin movement in corn coleoptiles. Planta (Berl.) 82, 123–144 (1968).

    Google Scholar 

  • Hollaender, A. (ed.): Hormonal control of protein synthesis. J. cell. comp. Physiol. 66, Suppl. 1, 1–182 (1965).

  • Ingle, J., K. W. Joy, and R. H. Hageman: The regulation of activity of the enzymes involved in the assimilation of nitrate by higher plants. Biochem. J. 100, 577–588 (1966).

    Google Scholar 

  • Key, J. L.: Ribonucleic acid and protein synthesis as essential processes for cell elongation. Plant Physiol. 39, 365–370 (1964).

    Google Scholar 

  • —, and J. Ingle: Requirement for synthesis of DNA-like RNA for growth of excised plant tissue. Proc. nat. Acad. Sci. (Wash.) 52, 1382–1388 (1964).

    Google Scholar 

  • —, and J. C. Shannon: Enhancement by auxin of RNA synthesis in excised soybean hypocotyl tissue. Plant Physiol. 39, 360–364 (1964).

    Google Scholar 

  • Masuda, Y., E. Tanimoto, and S. Wada: Auxin-stimulated RNA synthesis in oat coleoptile cells. Physiol. Plantarum (Cph.) 20, 713–719 (1967).

    Google Scholar 

  • Nooden, L. D., and K. V. Thimann: Evidence for a requirement for protein synthesis for auxin-induced cell enlargement. Proc. nat. Acad. Sci. (Wash.) 50, 194–200 (1963).

    Google Scholar 

  • ——: Inhibition of protein synthesis and auxin-induced growth by chloramphenicol. Plant Physiol. 40, 193–201 (1965).

    PubMed  Google Scholar 

  • ——: Action of inhibitors of RNA and protein synthesis on cell enlargement. Plant Physiol. 41, 157–164 (1966).

    PubMed  Google Scholar 

  • Ray, P. M., and A. W. Ruesink: Kinetic experiments on the nature of the growth mechanism in oat coleoptile cells. Develop. Biol. 4, 377–397 (1962).

    Google Scholar 

  • Schimke, R. T.: Protein turnover and the regulation of enzyme levels in rat liver. Nat. Cancer Inst. Monogr. 27, 301–314 (1967).

    PubMed  Google Scholar 

  • —: Regulation of protein degradation in mammalian tissue. In: Mammalian protein metabolism (H. N. Munro, ed.), vol. 4. New York: Acad. Press 1969 (in press).

    Google Scholar 

  • Seitz, K., and A. Lang: Invertase activity and cell length in lentil epicotyls. Plant Physiol. 43, 1075–1085 (1968).

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Biology, Kalamazoo College, Kalamazoo, Michigan

    Michael L. Evans & Russell Hokanson

  2. Longwood Program, University of Delaware, 19711, Newark, Delaware, USA

    Russell Hokanson

Authors
  1. Michael L. Evans
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Russell Hokanson
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Evans, M.L., Hokanson, R. Timing of the response of coleoptiles to the application and withdrawal of various auxins. Planta 85, 85–95 (1969). https://doi.org/10.1007/BF00387663

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00387663

Keywords

Search

Navigation