Abstract
We have previously selected structural variants of the Kk antigen from a (C3 × D2)F1 T-cell lymphoma. Those mutants were identified by the loss of certain epitopes defined by monoclonal antibodies. The variant Kk molecule from HK13.S3 cells is no longer recognized by 40% of the trinitrophenyl-specific, Kk-restricted cytotoxic T lymphocytes. Here we report on the primary structure of the altered Kk molecules from the cell line HK13.S3. Comparison with the parental Kk reveals a single base pair exchange, GCG to GTG, that results in an alanine to valine exchange in position 40 of the protein. This observation emphasizes that minor structural alterations in class I molecules may have a strong effect on the H-2-restricted T-cell response.
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Karmann, G., Beyreuther, K.T., Cramer, M. et al. A structural somatic variant of the Kk antigen is generated by point mutation. Immunogenetics 22, 35–48 (1985). https://doi.org/10.1007/BF00430592
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DOI: https://doi.org/10.1007/BF00430592