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The role of brush border enzymes in tubular reabsorption of disaccharides

A microperfusion study in rat kidney

  • Transport Processes, Metabolism and Endocrinology; Kidney, Gastrointestinal Tract, and Exocrine Glands
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Summary

Renal tubular reabsorption of maltose, sucose and lactose were studied in vivo et situ by continuous microperfusion of single proximal convolutions of rat kidney. The14C-label of maltose (2.5 mmol/l) was removed from the lumen of the proximal tubule at about the same rate as found for glucose. Maltose reabsorption was completely inhibited in presence of 30 mmol/l glucose or of 0.1 mmol/l phlorizin. Chemical analysis of the samples showed a complete conversion of maltose into glucose within a perfusion distance of 2 mm. It is concluded from these results that within the tubular lumen maltose is split very rapidly by a brush border glucosidase. The short half time of this process permits the breakdown product glucose to be almost completely reabsorbed subsequently within the proximal tubule.

In contrast, sucrose and lactose were neither split nor reabsorbed by the tubule brush border.

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Authors and Affiliations

  1. Institut für Physiologie der Universität, Fritz-Pregl-Strasse 3, A-6010, Innsbruck, Austria

    Stefan Silbernagl & Gertraud Vetter

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  1. Stefan Silbernagl
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  2. Gertraud Vetter
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Supported by grant No. 1740 of the Austrian “Fonds zur Förderung der wissenschaftlichen Forschung”

Parts of this study were reported at the 48th meeting of the German Physiological Society [29] and at the 2nd European Colloquium on Renal Physiology [31]

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Silbernagl, S., Vetter, G. The role of brush border enzymes in tubular reabsorption of disaccharides. Pflugers Arch. 371, 141–145 (1977). https://doi.org/10.1007/BF00580782

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  • DOI: https://doi.org/10.1007/BF00580782

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