Abstract
The dependence of the Ca2+-ATPase activity of sarcoplasmic reticulum vesicles upon the intravesicular concentration of calcium accumulated after active uptake was studied. The internal calcium concentration was modified by addition of the ionophore A23187 at the steady state of accumulation. About half of the calcium accumulated could be released at low ionophore concentration without any concomitant activation of the Ca2+-ATPase. This population of calcium might consist of calcium free in the lumen of the vesicles or bound to the bilayer at sites which do not interact with the ATPase activity. At higher concentrations of ionophore (above 1.75 nmol A23187/mg protein) the release of calcium activated this enzyme. This phenomenon was independent of the extravesicular calcium concentration and might be explained by assuming second species of calcium ions bound to the inner side of the membrane and in close functional interaction with the Ca2+-ATPase.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Arrio, B., Chevallier, J., Jullien, M., Yon, J., and Calvayrac, R. (1974).J. Membr. Biol. 18, 95–112.
Arrio, B., Tenu, J. P., and Chevallier, J. (1977).Biol. Cell 30, 111–118.
Berman, M. C. (1982).Biochim. Biophys. Acta 694, 95–121.
Bréthes, D., Chevallier, J., and Tenu, J. P. (1979).Biochimie 61, 109–113.
Chevallier, J., Bonnet, J. P., Galante, M., Tenu, J. P., and Gulik-Krzywicki, T. (1977).Biol. Cell. 30, 103–110.
Diamond, E. M., Norton, K. B., McIntosh, D. B., and Berman, M. C. (1980).J. Biol. Chem. 255, 11351–11356.
Dupont, Y. (1980).Eur. J. Biochem. 109, 231–238.
Gerdes, V., and Moller, J. V. (1983).Biochim. Biophys. Acta 734, 191–200.
Hasselbach, W. (1964).Prog. Biophys. Mol. Biol. 14, 167–222.
Hasselbach, W. (1974).The Enzymes 10, 431–467.
Ikemoto, N. (1976).J. Biol. Chem. 251, 7275–7277.
Lowry, O. M., Rosenbrough, N. J., Farr, A. L., and Randall, R. J. (1951).J. Biol. Chem. 193, 265–275.
McLennan, D. H., and Holland, P. C. (1975).Annu. Rev. Biophys. Bioeng. 4, 377–404.
Malan, N. T., Sabbadini, R., Scales, D., and Inesi, G. (1975).FEBS Lett. 60, 122–125.
Meissner, G., Conner, G. E., and Fleischer, S. (1973).Biochim. Biophys. Acta 298, 246–269.
Pfeiffer, D. R., Taylor, R. W., and Lardy, H. A. (1978).Ann. N.Y. Acad. Sci. 357, 402–422.
Sandow, A. (1970).Annu. Rev. Physiol. 32, 87–138.
Scarpa, A., Baldassore, J., and Inesi, G. (1972).J. Gen. Physiol. 60, 735–749.
Shigekawa, M., and Dougherty, J. P. (1978).J. Biol. Chem. 253, 1458–1464.
Tada, M., Yamamoto, T., and Tonomura, Y. (1978).Physiol. Rev. 58, 1–66.
Tenu, J. P., Ghelis, C., and Chevallier, J. (1974).Biochimie 56, 791–793.
Vale, M. G. P., and Carvalho, A. P. (1975).Biochim. Biophys. Acta 413, 202–212.
Yamamoto, T., Takisawa, H., and Tonomura, Y. (1979). InCurrent Topics in Bioenergetics (Sanadi, D. R., ed.), Vol. 9, Academic Press, New York, pp. 179–236.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Dulon, D., Bréthes, D. & Chevallier, J. Quantitative determination of the calcium involved in the regulation of the Ca2+-ATPase activity in sarcoplasmic reticulum vesicles. J Bioenerg Biomembr 19, 505–514 (1987). https://doi.org/10.1007/BF00770033
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF00770033