Summary
The third component of the pig complement system (C3) was isolated in hemolytically active form and characterized. The C3 component is a β-globulin with the molecular weight of 191,000 and is composed of 2 non-identical polypeptide chains of Mt 112,000 and 74,000. The isolated C3 can be used for the detection of the C3b receptor on the membranes of heterologous peritoneal macrophages.
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Hartmann, K. U., and Bokisch, V. A., J. exp. Med.142 (1975) 600.
Klaus, G. G. B., Humphrey, J. H., Kunkel, A., and Dogworth, D. W., Immun. Rev.53 (1980) 3.
Griffin, F. M., Bianco, C., and Silverstein, E. C., J. exp. Med.141 (1975) 1269.
Wellek, B., Hanh, H. H., and Opferkuch, W., J. Immun.114 (1975) 1643.
Lachmann, P. J., Behring Inst. Mitt.63 (1979) 25.
Müller-Eberhard, H. J., in: Progress in Immunology IV, p. 1001. Academic Press, New York 1980.
Tack, B. F., and Prahl, J. W., Biochemistry15 (1976) 4513.
Jacobse-Geels, H. E. L., Daha, M. R., and Horzinek, M. C., J. Immun.125 (1980) 1606.
Daha, M. R., Stuffers-Heiman, M., Kijlstra, A., and Vanes, L. A., Immunology36 (1979) 63.
Giclas, P. C., Keeling, P. J., and Henson, P. M., Molec. Immun.18 (1981) 113.
Paques, E. P., Hoppe-Seyler's Z. physiol. Chem.361 (1980) 445.
Dierich, M. P., Scheiner, O., Mussel, H. H., Hamman, K. P., Schopt, R. E., and Schulz, T., Molec. Immun.19 (1982) 1255.
Tiselius, A., Hjertén, S., and Levin, O., Archs Biochem. Biophys.65 (1956) 132.
Tack, B. F., Janatova, J., Thomas, M. L., Harrison, R. A., and Hammer, C. H., in: Methods in Enzymology 80, p. 64. Eds S. P. Colowick and N. O. Kaplan. Academic Press, New York 1981.
Laemmli, U. K., Nature, Lond.227 (1970) 680.
Yphantis, D. A., Biochemistry3 (1964) 297.
Ouchterlony, O., Prog. Allergy5 (1958) 1.
Laurell, C. B., Analyt. Biochem.15 (1966) 45.
Jessen, T. E., Barkholt, V., and Welinder, K. G., J. immun. Meth.60 (1983) 89.
Rossmann, P., Větvička, V., and Zídková, J., Cell Biol. int. Rep.7 (1983) 1041.
Berger, M., and Fleischer, T. A., J. Immun.130 (1983) 1021.
Větvička, V., and Viklický, V., Immunobiology163 (1982) 22.
Michl, J., Pieczonka, M., Unkelles, J. C., and Silverstein, S. C., J. exp. Med.150 (1979) 607.
Griffin, F. M., and Mullinax, P. J., J. exp. Med.154 (1981) 291.
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Zídková, J., Větvička, V., Rossmann, P. et al. Characterization of the third component of pig complement and its utilization in a C3b receptor study. Experientia 40, 975–977 (1984). https://doi.org/10.1007/BF01946467
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DOI: https://doi.org/10.1007/BF01946467