Genome-wide screen in human plasma identifies multifaceted complement evasion of Pseudomonas aeruginosa
Fig 4
ATP and biotin influence bacterial sensitivity to plasma.
A. Schematic view of the purine pathway (adapted from KEGG database [42]). Significantly enriched insertions in corresponding genes of the Tn-seq screen are indicated. B. Measurement of intra-bacterial ATP levels in LB and after 2h-incubation in HIP normalized to the CFU counts. C. Trans-complementation of ΔbioB and ΔpurD phenotype by exogenous biotin or ATP, respectively. Biotin (1 μM) and ATP (1.5 mM) were added at the beginning of the incubation of bacteria in plasma. The survival was estimated by CFU counting and the median of all independent experiments is represented by the histogram. Statistical analysis was performed and p-value <0.05 or 0.01 are indicated with ‘*’ and ‘**’, respectively. D. The biotin biosynthetic pathway. Significantly enriched hits in Tn-seq are highlighted in green.