Hypermutation-induced in vivo oxidative stress resistance enhances Vibrio cholerae host adaptation
Fig 4
Rugose phenotypes of V. cholerae isolated from NAC- and NAC+ mice.
Fecal pellets from 5-day-PI NAC- and NAC+ mice were resuspended in PBS and diluted samples were spread onto selective LB plates. After overnight incubation at 37°C, the plates were incubated at room temperature for two days. The colonies were photographed (A) and the percentage of rugose colonies was determined (B). Each data point represents the percentage of rugose colonies out of at least 300 total colonies isolated from one mouse. Horizontal line: average percentage of 8 mice. ****: One-way ANOVA test, P value <0.0001. C. Reversion rate of the ΔmutS* rugose variants. Rugose colonies were resuspended in LB and spread onto selective LB plates. After overnight incubation at 37°C aerobically (circles) and anaerobically (squares), the plates were incubated at room temperature for two days. The percentage of smooth colonies was determined out of at least 400 colonies. Colors correspond to unique isolates. ***: Student t-test P < 0.001.