Infantile restrictive cardiomyopathy: cTnI-R170G/W impair the interplay of sarcomeric proteins and the integrity of thin filaments
Fig 3
Ca2+-dependent activation of reconstituted thin filaments.
Activation of thin filaments with troponin complexes containing cTnI variants: wildtype (black, solid line), R170G (gray, dotted line) and R170W (white, dashed line), measured by PM-tropomyosin fluorescence in presence of (A) myosin S1 and ATP as well as (B) cMyBPC-C0C2. Data are given as normalized fluorescence ±SEM (n = 5) vs. the negative logarithm of the Ca2+-concentration (pCa), fitted to the Hill equation to determine Ca2+-sensitivity (pCa50) and the cooperativity (Hill coefficient nH).