Safety and vaccine-induced HIV-1 immune responses in healthy volunteers following a late MVA-B boost 4 years after the last immunization
Fig 3
Frequency, function and phenotype of HIV-specific T cell responses.
A: Percentage of responders with positive ICS responses against Env+Gag+GPN at the different time points (left panel) and distribution of the CD4+ and CD8+ T cells by antigen (right panel). B: Functional profile of vaccine-induced T cells. The quality of the HIV-specific CD4 or CD8 T cell response is characterized by the proportion of cells making every possible combination of the measured cytokines: IFN-γ (I); IL-2 (2); TNF-α (T) and CD107a (C). Responses are grouped and colour coded on the basis of the number of functions. The bar charts show the mean values and interquartile ranges (IQR) and the pie charts show the average proportion of the HIV-specific CD4 or CD8 T cell responses according to the functions at weeks 0, 2, 4 and 12. “+”distributions that are different from the earliest time point (W0) within each category at p<0.05 (Student's T test). C: Phenotype of vaccine-induced T cells. The graphic represents the distribution of the responding HIV-specific CD4 and CD8 T cells at any time point based on CCR7 expression in combination with CD45RA within the Naïve (CD45RA+ CCR7+), T central memory (TCM: CD45RA- CCR7+), T effector memory (TEM: CD45RA- CCR7-) or terminally differentiated T effector memory (TEMRA: CD45RA+ CCR7-) phenotypes. Statistical differences were determined using ANOVA test (using the linear model y ~ x_patient + x_cellType + epsilon) followed by Tukey's honest significant difference criterion. *p<0.05, ***p<0.005.