Characterization and expression analysis of Galnts in developing Strongylocentrotus purpuratus embryos
Fig 7
SpGalNAc-T13 is required for embryo skeleton and nerve cell development.
(A-B) DIC images of 3-day embryos showing skeletal spicules in the control embryo (A, arrowheads) compared to a lack of spicules in the SpGalnt13 morphant (B). (C-H) fluorescent images of expression patterns of different markers. (C-D) Primary mesenchyme cell (PMC) antibody marker 6a9 (red) was detected in both control embryos (C) and SpGalnt13 morphants (D), indicating that PMC cells ingressed in the morphant but could not form spicules. (E-F) Oral ectoderm marker Goosecoid (GSC, red) was detected in both the control embryo (E) and the SpGalnt13 morphant (F), but ciliated band ectoderm marker Hnf6 (green) was not detected in the SpGalnt13 morphant. (G-H) Serotonergic neuron marker (green) and pan-neuronal marker Synaptotagmin B (SynB, red) were detected in the control embryo (G) but absent in the SpGalnt13 morphant (H). All embryos are shown in oral view, with the anterior pole to the top. Scale bar in (A) indicates 20 μm. Representative embryos are shown from three separate experiments.