ABRAXAS (FAM175A) and Breast Cancer Susceptibility: No Evidence of Association in the Breast Cancer Family Registry
Fig 3
Functional assays assessing the impact of variant rs145796091 c.21G>A (p.Ser7Ser) on transcriptional activity and splicing efficiency in the MCF7 breast cancer cell line.
A) Gene reporter assays. Luciferase activity was normalized to Renilla luciferase. Each experiment was performed three times. Data from four replicates per construct were analyzed with a mixed model including the fixed effect of genotype and the random effects of experiment number and clone within experiment number. B) Splicing reporter mini-gene assays. Mini-gene constructions: exons 1, 2 and 3 of ABRAXAS were subcloned in a pcDNA3.1 vector. C) Gel-electrophoresis of mini-gene RT-PCR products on 2% agarose gels.