ANKS1B Interacts with the Cerebral Cavernous Malformation Protein-1 and Controls Endothelial Permeability but Not Sprouting Angiogenesis
Fig 3
ANKS1A does not affect endothelial proliferation, migration and capillary formation.
(A) Representative Western blot for HUVEC protein lysates probed with anti-ANKS1A antibody after transfection with non-silencing control siRNA or a pooled set of ANKS1A siRNAs. GAPDH served as protein loading control. (B) Fold change of mRNA transcript levels of ANKS1A in HUVEC after transfection with non-silencing control siRNA or smart pool of ANKS1A siRNAs. This had no effect on proliferation of endothelial cells measured by BrdU incorporation (C) and migration (D). (E) Sprouting of HUVEC spheroids in a collagen matrix was not altered after ANKS1A knockdown. *, p < 0.05; **, p < 0.01, n = 3 experiments. Bar graphs show mean values, error bars indicate SD.