T Cells Detect Intracellular DNA but Fail to Induce Type I IFN Responses: Implications for Restriction of HIV Replication
Figure 4
Expression of DNA signaling pathway molecules in IL2/PHA PBMCs and CD4+ T cells.
(A) Whole cell lysates of IL2/PHA PBMCs from 2 donors were analyzed for expression of IFI16, STING, TBK1, IRF3 and β-actin by Western Blotting. (B, C) Whole cell lysates from IL2/PHA-treated PBMCs and CD4+ T cells were stimulated with ssDNA, dsDNA (both 2 µg/mL) or lipofactamine for 2 h of IL2/PHA PBMCs were analyzed for levels of IFI16 and cGAS by Western Blotting. Similar results were obtained with two independent donors.