Trypanosoma cruzi Infection in Neotropical Wild Carnivores (Mammalia: Carnivora): At the Top of the T. cruzi Transmission Chain
Figure 2
Trypanosoma cruzi genotyping of wild carnivore isolates from the Pantanal wetland, Brazil.
Representative agarose electrophoresis gels stained with ethidium bromide of the (A and B) Mini-exon multiplex PCR products, (C) 1f8 gene/Alw21I PCR-RFLP products and (D) kDNA PCR products. Lanes: M. Molecular weight markers (100bp DNA ladder), 1- Procyon cancrivorous isolate; 2–17– Nasua nasua isolates. Control samples: TcI, TcII, TcIII/TcIV, TcV/TcVI, TR - T. rangeli, TC – T. cruzi and C- Negative control.